FastQCFastQC Report
Thu 28 Apr 2016
ERR1041925.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041925.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3087730
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT154730.501112467735197No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT119950.3884730854057835No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75730.24526108176556888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45870.14855573511932715No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT37890.12271150651125583No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC34160.11063143474332275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG1600.020.812535
ACGGACC8950.017.3631298
GACGGAC8750.017.1257157
CGCAAGA8850.016.9322032
AAGACGG9600.016.5729165
CGGACCA9500.016.3578979
ATTAGAG6450.016.0620163
AGACGGA9600.015.9947916
CGAACGT3250.015.9384614
TATACTG4100.015.3414635
TCGTTTA5450.015.27522930
CGAACGA4250.015.23529416
TAACGGC2201.8189894E-1215.13636436
TAGAGTG6000.015.1083345
TCGAACG3500.014.83
TAACCGG908.2795846E-414.38888922
TTAGAGT6200.014.322584
CAAGACG11400.014.2807034
TAACGAA4600.014.07608713
ACACGCT9900.014.015159
Produced by FastQC (version 0.11.5)