FastQCFastQC Report
Thu 28 Apr 2016
ERR1041924.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041924.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1468607
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT130710.8900270800833715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT92250.6281462637724047No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45370.30893220582497566No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36910.2513265972448722No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT28240.19229106221065267No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC25410.17302110094804124No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA19330.13162132551458627No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC2600.021.3461532
TCGTTTA3000.018.530
TACGGGT500.007035790518.54
CAAGACG6900.017.9637684
AAGACGG6700.017.9477625
AGGTTAT3200.017.9218751
CGCATCG3150.017.61904713
ACGGACC6450.017.4961228
GACGGAC6400.017.343757
TAACGCC3550.017.1971844
TAATCCG650.001580216817.0769235
CGAACGT1655.4569682E-1116.8181824
GCGAAAG6650.016.69172918
GCGTAGA1005.880027E-616.6500021
TTAGAGT2450.016.6122464
CGAAAGC6700.016.56716319
GAGCGAA6750.016.44444516
ATGGTCG3150.016.44444536
CGGACCA6750.016.4444459
TTATCTA3500.016.3857144
Produced by FastQC (version 0.11.5)