FastQCFastQC Report
Thu 28 Apr 2016
ERR1041922.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041922.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2088244
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81890.3921476609055264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60950.29187202261804657No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT30490.14600784199547562No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27540.13188114032651357No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22610.10827278804584138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTA3850.018.7402630
ATTAGAG3650.017.7397253
TACGACG3800.017.5263165
AAGACGG7500.017.0199995
CGTTTAT4550.016.26373531
TTAGAGT3450.016.0869564
CGACGGT3800.016.065797
CAAGACG7900.015.6898734
CGCAAGA7350.015.6054432
ACGACGG4050.015.5308646
ACGGACC7350.015.3537428
TATACTG3900.015.1794875
GACGGAC7450.015.1476517
CGTGACT1001.0940434E-414.79999912
GCGCAAG8500.014.5823541
TAGAGTG3300.014.5757575
CGAACGA2950.014.42372916
ACGAACG2950.014.42372915
ATGGTCG4900.014.34693836
AGACGGA8300.014.0421696
Produced by FastQC (version 0.11.5)