FastQCFastQC Report
Thu 28 Apr 2016
ERR1041921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999318
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39390.19701718285935502No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG36160.18086167383077628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT24500.12254178674928151No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA24110.12059112157245622No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGA22750.11378880198147569No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAAT22480.11243834147444279No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT22390.1119881879720985No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC20720.10363533965082093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20340.10173469152981167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG450.003826783520.5555555
TCTAGCG4000.018.528
GTACTAG1156.411938E-817.6956521
CTAGCGG4200.017.61904729
GCGCATT953.609086E-617.52631611
GTTCAAA7700.017.0584431
ATTAGAG2500.017.023
CGAGCCG6900.016.89130415
TTAATAC1352.2218956E-816.4444433
ATACCGC6900.016.35507227
GACGGAC5000.016.2799997
GATTATA1251.6593913E-716.2799991
TACCGCA6500.016.22307828
CGCAATA4750.015.96842136
AGGCCCG7450.015.89261710
TCGTTTA3850.015.85714430
ATAATAC2100.015.8571423
ACGGACC5150.015.8058268
TGGATAC7350.015.60544224
TATTAGA1802.0190782E-1015.4166662
Produced by FastQC (version 0.11.5)