FastQCFastQC Report
Thu 28 Apr 2016
ERR1041920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3610010
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT75930.21033182733565833No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60310.16706324913227388No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT58810.16290813598854298No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACC12550.018.8685268
AAGACGG14350.017.919865
CGCAAGA13950.017.9032252
CGAACGT3850.017.779224
GACGGAC13450.017.6059477
CGTCGTA9150.017.59016410
GGTTATC6850.017.5547452
ACCGTCG9700.016.9742268
CAAGACG15300.016.807194
ATACCGT11000.016.6500026
TTATCTA7900.016.6265814
CCGTCGT9800.016.6122469
TACCGTC10400.016.543277
GCGCAAG15100.016.4172171
AGACGGA15150.016.3630376
CGGACCA14700.016.3605449
TCGAACG4100.016.2439043
GCGAAAG15000.015.78666618
TCGTTTA6100.015.77049330
GCAAGAC18250.015.408223
Produced by FastQC (version 0.11.5)