FastQCFastQC Report
Thu 28 Apr 2016
ERR1041919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8816793
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT828770.9399903116700142No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT693680.7867713351101698No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT461080.5229565897713602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT306990.348187827478767No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG212860.24142565216173276No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG140910.15982001618956007No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG127030.1440773306121625No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT100330.11379421066140488No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT9350.018.00534630
CGCTATC10100.016.66831833
GACGCTA10700.016.59813126
CGAGACG12050.016.27385923
ACGCTAC11050.015.73755627
ATCTCGT10600.015.70754737
GCTACGC11100.015.66666729
CCCACGA16250.014.91384619
CGCTACG11400.014.60526328
ATCTCCG18300.014.45628410
TATACAC22050.014.430843
CGAGCCC18300.014.3551915
TCTCCGA18850.014.32891311
CACGAGA16250.013.88923121
TCCGAGC19550.013.62659913
TATCTCG12500.013.3236
ACGAGAC15550.013.08681822
GCCCACG19250.012.97402518
TACACAT25800.012.8352725
CTAGTAC6350.012.5275593
Produced by FastQC (version 0.11.5)