FastQCFastQC Report
Thu 28 Apr 2016
ERR1041918.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041918.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7949156
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100480.12640335653244195No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC84940.10685411130439508No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT81450.10246370809680928No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64000.020.4656261
TATTAGA28000.015.1964292
ATTAGAG25550.015.1330733
TTAACGG22950.014.75163435
TAACGGC24700.013.70647736
GTATCAA96750.013.4614992
GTATTAG39050.013.312421
CGTATTA5100.013.05882415
TACCCCG13950.012.8637995
GTTTAAC28050.012.59714733
TTTAACG27800.012.57733834
TTAGGAC19650.012.4274823
TAATACT29200.012.3544534
TCTAATA25250.012.3089112
ACCGCCT30250.012.292562512
TAGGACC19500.012.143594
ATCCGTA5700.012.00877212
GGGGTTA15150.011.9669956
GGCACCG31200.011.9182699
TAGGGGT15400.011.8928574
Produced by FastQC (version 0.11.5)