FastQCFastQC Report
Thu 28 Apr 2016
ERR1041916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3667128
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT324600.885161357880063No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT283370.7727300492374414No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG224290.6116230467003061No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG193540.5277699605795052No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT134230.36603576422748263No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG122430.3338579945941347No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA104520.28501868492182436No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA95970.2617034365857968No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG87030.23732468569409088No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA85490.23312521406397596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT83990.22903481961905883No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT80640.21989960535874395No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG80490.21949056591425223No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT80320.21902698787716166No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC71630.1953299693929418No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG59500.1622523129817121No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT52630.14351830642399174No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52040.14190941794232434No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT51330.13997329790506358No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG48390.13195612479302604No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT43110.11755793634691782No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG39290.10714106516052889No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT39060.10651387134564161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC11750.024.0893613
CTTATAC14200.019.281691
TTATACA14400.019.013892
ATACACA17850.016.6862744
CGCGTAA1005.8848564E-616.65000210
ATCTCCG15950.016.58620810
ATCTCGT14450.016.25951637
TACACAT17000.016.105885
TCTCCGA16500.015.92121311
ACACATC17400.015.7356336
AGACCGA16500.015.58484925
CCCACGA16650.015.55555519
ACCGAGG17250.015.44347827
CACGAGA17200.015.38081421
GAGACCG17350.015.1412124
ACGAGAC17250.015.12173822
GACCGAG17850.015.0280126
TTAGCGA750.004106686514.827
TCCGAGC18500.014.813
GCCCACG17900.014.7793318
Produced by FastQC (version 0.11.5)