FastQCFastQC Report
Thu 28 Apr 2016
ERR1041915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9146723
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT893170.9764917993034226No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT662330.7241172603565234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT463240.5064546067482311No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG449260.4911704443219719No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341610.37347802048886797No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG279850.3059565704569822No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG260740.285063841990186No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT199820.21846075364914844No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT192050.20996590800880272No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG159690.17458711715660352No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA141250.15442689146703142No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG135240.14785623222655808No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT125580.13729507278180392No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA119510.13065881627769857No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG109340.11954008009207233No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108040.11811880604671203No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA107370.11738630326948787No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT103830.1135160647151991No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA99480.10876026310187813No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG99370.10864000145188611No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG97450.10654088901566167No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT96580.10558972869299749No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC96140.1051086820930294No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC95750.10468229987942129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT13050.021.26436830
CGAGACG15100.021.0728523
CGCTATC14250.019.3438633
ATCTCGT15400.019.10064937
TATACAC22700.018.9889873
GACGCTA16100.018.95962726
CGAGCCC22200.018.08333415
TCTCCGA22650.018.05077411
TATCTCG16050.017.9813136
CGCTACG16250.017.87384628
CCCACGA21400.017.63551319
TACGCTA15650.017.49520931
ACGCTAC17200.017.3168627
GCTACGC16950.017.24483929
ACGAGAC19100.017.24083722
ACGCTAT16250.017.1907732
ATCTCCG23850.017.06499110
GCCCACG23750.016.20210618
GCTATCT18250.015.81369834
TTAACGG13400.015.73880635
Produced by FastQC (version 0.11.5)