FastQCFastQC Report
Thu 28 Apr 2016
ERR1041914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8339787
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT603110.7231719467175841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT474460.5689114122459002No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT286160.3431262692920095No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218810.2623688110979333No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG172530.20687578711542634No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG111350.133516599404757No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG99160.11889991914661609No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA8450.014.01183325
CGAGACG9150.013.34426123
CTAGTAC5150.013.2912623
GGGTAAG9500.012.6578951
GACGTAG9600.012.52604126
ACGTAGA9950.012.45728827
TATACAC15250.012.3737713
TATACTG11300.012.2787615
CGTAGAG10000.012.02528
TATACCG3550.011.9859155
TAATACT23650.011.7336154
CTAGACT8450.011.603554
TTAGACT6700.011.5970154
CTACACT14650.011.3651894
GTACTAG5250.011.276191
TAGGGGT11650.011.2746784
ATACCGT6100.011.2213126
CGTATTA4150.011.14457815
GAGACGT11200.011.06696424
TCTAATA19400.011.0618552
Produced by FastQC (version 0.11.5)