FastQCFastQC Report
Thu 28 Apr 2016
ERR1041913.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041913.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2571615
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT356831.3875716232795345No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT293621.141772777029221No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG183690.7142982133795299No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG180550.7020879875097944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167350.6507583755733265No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG120270.46768275966659084No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA101940.3964045940002683No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA97840.38046130544424417No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA85320.33177594624389734No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG85300.3316981741045996No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT75680.2942897751024162No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG72140.28052410644672704No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT68940.2680805641590985No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66660.2592145402791631No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC65950.2564536293340955No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT62420.24272684674805522No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT50540.19653019600523405No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT45400.17654275620573065No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG44160.17172088356927456No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT39670.1542610382969457No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG37240.14481172337227774No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG36760.14294519202913344No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT35090.13645121839777727No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT34010.1322515228757026No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC32690.12711856168205585No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT28480.11074752635989446No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA27980.1088032228774525No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG27290.10612008407168258No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC8550.019.4736843
TATACCG904.4493987E-516.4444455
ATCTCCG8650.016.25433510
TATATCG803.3846032E-416.18755
ACGAGAC8750.016.06857122
ACCGATC700.00259366315.8571438
TCTCCGA8750.015.85714311
GCCCACG9100.015.85714218
CCCACGA9000.015.62222219
TATACTG3100.015.51612955
ATAGAAC2055.456968E-1215.3414633
ATCTCGT8200.015.11585337
AGACCGA9350.015.03743425
ACACCGC1853.0559022E-1015.06
CGAGCCC9500.014.99473715
GCTCCGA3100.014.91935529
TACACAT11250.014.85
GAGACCG9450.014.6825424
ACCGAGG9200.014.67934827
TTATACA10000.014.6152
Produced by FastQC (version 0.11.5)