FastQCFastQC Report
Thu 28 Apr 2016
ERR1041911.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041911.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13418583
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT310660.2315147583019757No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT277250.20661645123035716No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG222140.16554654094251234No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT174160.1297901574257133No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC149800.11163622865394954No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG147360.10981785483608812No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG137390.10238786017867907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG41150.014.83596535
TAACGGC42600.014.37441436
TATTAGA52450.014.0734032
ATTAGAG48800.013.950823
TATACAC17900.013.8491613
GTATTAG67100.013.7853961
GGGGTTA29050.013.6919116
TAATACT52400.013.274814
CTCTAAT40800.013.1495091
TTTAACG49400.013.0323934
GGGTAAG19200.013.0078121
AATACTG45650.012.9682375
GTTTAAC49850.012.91474433
TCTAATA45000.012.8677782
AACGGCC48900.012.74948937
CTAGTAC8150.012.7116573
GTGACAC50950.012.63591824
GGTAAAC37550.012.51398235
CACATGT52400.012.39217628
GGGTTAG31250.012.3136017
Produced by FastQC (version 0.11.5)