FastQCFastQC Report
Thu 28 Apr 2016
ERR1041910.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041910.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12531849
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT508020.40538311625044315No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT504890.4028854800277278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT327400.2612543448297215No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218580.17441959283103395No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG154110.12297467037785087No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG153480.12247195126593052No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC136340.10879479955431956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG35000.014.835
TCTAATA53550.014.4752572
TAACGGC35850.014.44909436
GGTAAAC44150.014.41449635
CTCTAAT48600.014.3508231
TAATACT61100.014.2610474
AATACTG55650.014.0287515
ATACTGG54350.013.6835326
ATGCTAG49500.013.60404115
GTATTAG57950.013.5358071
GGGTAAG20000.013.5050011
CTAATAC63750.013.4650983
TACTGGT52200.013.4319937
TAGGGGT31350.013.159494
GGGGTTA31050.013.107896
TATTAGA50550.013.101882
ATTAGAG44500.013.0539323
TAGGACC27800.013.0431654
GTAAACA49000.013.0255136
GATGCTA51400.012.92120614
Produced by FastQC (version 0.11.5)