FastQCFastQC Report
Thu 28 Apr 2016
ERR1041908.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041908.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10408258
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301120.2893087392722202No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT285120.2739363301716771No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT172590.16582025541642031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123130.1183002957843666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG11500.012.70869535
TAACGGC11950.012.23012436
GTATTAG29950.011.9833051
GGTATCA206750.011.7308351
TAATACT32350.011.4374044
TATACTG15000.011.3466665
TCTATAC13850.011.2202183
TAGGGGT14850.011.2121214
AATACTG29500.011.1627115
TAGTACT12500.010.8044
CTACACT21150.010.7588644
CTAATAC30150.010.7379773
ATTAGAG19300.010.7357513
CTCTAAT20200.010.5321791
TCTAATA23450.010.4925372
CGTATTA5500.010.42727315
CTAGCCT12550.010.3187254
CCGTTTA3452.9649527E-1010.18840513
TATTAGA24450.010.1390592
GTCTTAG24750.010.0909091
Produced by FastQC (version 0.11.5)