FastQCFastQC Report
Thu 28 Apr 2016
ERR1041906.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041906.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12250989
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1176670.9604693955728799No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG936240.7642158522875173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT727040.5934541284789334No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG623980.5093303079449341No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG557960.4554407811483628No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT506670.4135747734325776No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT457470.37341475043361805No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT425920.34766172755522023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT404460.3301447744341294No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG372740.3042529872486213No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA333550.27226373315656394No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG323470.2640358260055576No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA287270.2344871911973801No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA267140.2180558647142692No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT257660.21031771394129894No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG248740.2030366691211624No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC224110.18293216980278082No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT222730.18180573013329782No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG221790.18103844514104128No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC202590.16536624104388634No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG174330.14229871563838642No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG151010.12326351774538365No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149930.12238195626491868No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA132970.1085381759790985No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT131140.10704441902608844No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT124040.10124896855266134No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC28000.018.3678573
GTACTAG8700.015.5229891
ATCTCGT28100.015.33985737
ATCTCCG31650.014.90521310
AGACCGA30750.014.73983925
ACGAGAC31450.014.52941122
CCCACGA31500.014.5063519
TACACAT37800.014.437835
CTAGTAC9600.013.6822923
TCTCCGA34250.013.66569311
CGAGCCC34200.013.63157915
GAGACCG34000.013.43970624
ATACACA41050.013.3848974
TTAACGG14800.013.2535
TTATACA38900.013.221082
CACGAGA34900.013.19914121
GACCGAG35650.012.973351526
ACCGAGG36150.012.94744127
CTAATAC33450.012.5545593
GCCCACG36900.012.53387518
Produced by FastQC (version 0.11.5)