FastQCFastQC Report
Thu 28 Apr 2016
ERR1041904.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041904.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4265337
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT92990.21801325428682422No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG79880.18727711315659232No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT67680.15867444940458397No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64600.15145344904751956No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG64310.15077354966325052No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT63970.14997642624721094No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG60360.14151285115337897No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG52810.12381202235602953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47940.11239440166158031No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT46910.1099795866071075No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG1103.858986E-818.51
GTCGGGA10200.015.7794132
TATTAGA10900.014.596332
ATTTGCG11150.014.10313916
GGTCGGG12550.013.4143431
GGGGTTA6550.013.2748096
TTAACGG8400.013.21428735
GTACCGT850.00941019713.0588246
CGCTATA6750.012.8814812
ATTAGAG10500.012.8619043
CGAACTA7950.012.79874124
GTATTAA2501.6007107E-1012.5800011
CATACGG2951.8189894E-1212.54237333
GTGGGTA14200.012.5070439
GTAATTT13050.012.47509613
TAGAACT4200.012.3333344
GGTAAAC6500.012.238461535
CTCTTAT28800.012.20486237
CCATACG2901.2732926E-1112.12068932
TGCGCGC13150.012.09885919
Produced by FastQC (version 0.11.5)