FastQCFastQC Report
Thu 28 Apr 2016
ERR1041903.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041903.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393796
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGC45270.10303163824629091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA9500.016.5526312
GGTATCA91300.016.4737131
GTCGGGA12800.015.1757822
ATTAGAG10350.014.4782613
TTAGACT3100.014.3225814
TATACTG3500.014.2714285
CGTATAA1051.65731E-414.09523933
ATTAGAC2650.013.9622643
TTAACGG8350.013.95808435
TAATACT8750.013.9542864
TATCTCG9950.013.94472336
ATAATAC3750.013.8133343
TACCCCG6200.013.7258075
GTACAAA17150.013.4839651
ACTCTAA3650.013.17808210
GTATTAT3950.013.1139241
CTATACA2408.185452E-1113.1041664
GGACTTA16200.013.01851814
GCTATAC2001.4659236E-812.9500013
GGTCGGG15450.012.8122971
Produced by FastQC (version 0.11.5)