FastQCFastQC Report
Thu 28 Apr 2016
ERR1041902.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041902.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42588466
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1193990.28035524923579075No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1161450.2727146828909029No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT899370.2111768947019599No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT506860.11901344368684234No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT427960.10048730095138904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA145200.016.0282362
TATTAGA103850.014.2156952
GGTATCA1202950.014.2131411
GGTCGGG172600.013.6123991
TTAACGG83350.012.82903435
TAATACT113700.012.46354
TATACTG38950.012.3966615
GTGGGTA196950.012.3709069
GTACTAT53650.012.3448271
GGGAGTG221300.012.00451855
GTATTAG188900.011.9970881
CTCTTAT350850.011.97477537
ATTTGCG194850.011.87759716
TAACGGC90350.011.87603736
TCTAATA87200.011.753442
TCTTATA235300.011.5733137
ACCGCCT111000.011.56666612
TTTGCGC200550.011.42931817
ATTAGAG133250.011.4262663
AATACTG101400.011.2933925
Produced by FastQC (version 0.11.5)