FastQCFastQC Report
Thu 28 Apr 2016
ERR1041901.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041901.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38127556
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1519660.39857262290821893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1486430.3898571416431727No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1152840.3023639910200381No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT423360.11103780163617097No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA781500.018.038391
TTAACGG80900.014.45241135
TATTAGA111150.013.9478192
GGGGTTA73700.013.85617356
TAACGGC86150.013.55020336
ATTAGAG105350.013.4864753
TAATACT143350.013.408794
AATACTG112600.013.3574615
TCTAATA110500.013.2429882
GGGTTAG82300.012.6555297
GTATTAG182100.012.6482711
GGTAAAC87250.012.53123335
GTATCAA1124250.012.5094062
GTACTAT62450.012.2345881
ATGCTAG95300.012.15215115
ATACTGG114950.012.1187486
CTCTAAT105850.011.9546541
GGGTAAG53700.011.8510251
TACTGGT109650.011.6922037
TAGGGGT74450.011.6541314
Produced by FastQC (version 0.11.5)