FastQCFastQC Report
Thu 28 Apr 2016
ERR1041900.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041900.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4828330
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83320.17256484125981447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82470.17080439820807608No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT59580.12339670237949767No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG59250.12271323625352865No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT59020.12223688107482297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55910.11579573061493312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG15350.016.511430
ACTAGAT17300.015.29190732
TATACAC16550.015.2024173
TACACAT17650.015.0934845
ATCTCGT17050.014.97360737
CTAGATC17300.014.97109833
ATACACA19700.014.83756354
TATTAGA16700.014.7335332
ATCTCCG18400.014.67934810
GTATAAC2700.014.3888891
ATACCGT2201.8189894E-1114.2954546
GACCGTA17650.014.25495726
TCGCTAA2750.014.12727414
TATATCG3050.013.950825
ACATCTC20450.013.93154058
TAGATCT18800.013.77659634
TCTCCGA19300.013.611411
TACTAGA19350.013.57622731
AGACTAT4250.013.4941186
ATTAGAG16050.013.485983
Produced by FastQC (version 0.11.5)