FastQCFastQC Report
Thu 28 Apr 2016
ERR1041899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1318355
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT84570.6414812398784849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT82940.6291173469968256No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60150.4562504029643002No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.20419386280630028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAA250.00549663229.634
TTACGCT405.9407845E-527.754
GGTATCA36500.017.4863011
TCGCTAA852.7236072E-517.41176414
GTCGGGA3000.016.652
CATCTAG1403.473906E-815.8571431
TATCGTA700.002592764315.85714328
CGCCTTA957.0609334E-515.57894824
CAATACC957.0609334E-515.5789484
GTGCTAG1201.9359268E-615.4166671
TTCTAGC1701.4861143E-915.2352942
GGTCGGG3350.014.9104471
CGCAGTA1252.9593593E-614.79999918
ACGTCAA908.275155E-414.38888818
TTAACGG3100.014.3225835
TCTATAC1304.4448643E-614.2307693
CCGACGG1702.4369001E-814.14705919
TTCGGAC800.00629927113.8753
GTTATAC800.00629927113.8753
TAACGGC3250.013.66153836
Produced by FastQC (version 0.11.5)