FastQCFastQC Report
Thu 28 Apr 2016
ERR1041898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6186821
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT422140.6823213407984487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT420740.6800584662139086No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT321160.5191034296935373No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119590.19329797968940754No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATTAGA19550.015.9923272
GGTATCA244750.015.9564881
TCTAATA22050.015.1020412
TACTGGT18000.015.0055567
ACCGCCT17300.014.86416212
GGCACCG18050.014.7590039
TTAACGG15650.014.65814735
TAATACT26850.014.6070774
AATACTG22300.014.6008975
ATTAGAG20200.014.4702983
ATACTGG20050.014.1172076
TAACGGC16500.013.9030336
GTCGGGA12650.013.8932812
GGTAAAC17350.013.64841535
GACACAT19150.013.62140926
GTATTAG31700.013.5394321
TTGGTAA17850.013.4733933
ACCGCGT1102.4590897E-413.4545458
GATGCTA18850.013.44562314
ACACCGT3200.013.2968756
Produced by FastQC (version 0.11.5)