FastQCFastQC Report
Thu 28 Apr 2016
ERR1041897.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041897.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6083696
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT197310.3243258703261964No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT184290.3029244064792192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT144790.23799677038431902No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115300.018.0026021
TCGCTAA2353.6379788E-1214.17021414
TATACTG6800.013.3308825
TCTATAC4900.013.2142873
GGGGTTA9850.013.1472076
TTAACGG10500.013.038094535
TATTAGA15400.012.8538962
GTATCAA164400.012.6709252
TACTCCG18850.012.5623345
TAACGGC10900.012.38990936
ATTAGAG15650.012.293933
TAATACT20050.012.2718214
TCTAATA14700.012.2074832
CCGGTCT18250.012.1643839
GTACTAT7850.012.0191091
TCTAGAT11400.012.0087732
AATACTG16100.011.9503115
TCCGGTC18550.011.8679268
CTCTTAT42300.011.85224637
TACCCCG7700.011.7727275
Produced by FastQC (version 0.11.5)