FastQCFastQC Report
Thu 28 Apr 2016
ERR1041896.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041896.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998979
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT15050.15065381754771623No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT14310.14324625442576872No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT14110.1412442103387559No Hit
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG12350.12362622237304288No Hit
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT11970.11982233860771847RNA PCR Primer, Index 46 (95% over 21bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG11950.1196221341990172No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATA11830.1184209077468095No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT11660.11671917027284857No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT10970.10981211817265428No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC10200.10210424843765484No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG9990.10000210214629138No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT2900.021.05172336
CGATATG450.003825463620.55555516
CTTAAAC1550.020.2903231
TTATACA3600.020.0416662
ACCGTAT656.900975E-519.92307711
CCGTCTT3050.019.40983637
TATACAC3750.019.2400023
ATGTACC609.235664E-418.54
GTAAGAC852.7225371E-517.4117643
TACACTT752.066925E-417.2666665
CTTATAC4400.017.2386361
GTATATC650.001579738517.0769234
CGAACTA1452.9776857E-916.58620624
TAAACAC2250.016.4444453
CGTGTCG700.002592175315.8571429
TCGTTTA957.058198E-515.57894832
GCTCCGA1902.7284841E-1115.57894829
TATATCG750.004103905514.85
CTACCGT750.004103905514.89
GGTAAGA1405.992042E-714.5357142
Produced by FastQC (version 0.11.5)