FastQCFastQC Report
Thu 28 Apr 2016
ERR1041895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204070
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59810.4967319175795427No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56350.46799604674146855No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41270.3427541588113648No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20300.1685948491366781No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG502.702205E-422.229
TTAGACT759.266376E-619.7333324
TAACGGC2550.018.13725536
TTAACGG2550.018.13725535
ATACCGT953.606674E-617.5263166
ACCGTCG650.001579992817.0769238
ATTGTAC1005.8782553E-616.6500023
CGTATTG904.4462373E-516.44444527
CATACTG1059.343754E-615.8571445
GTTAGAT957.0601185E-515.57894827
TCTAGAT2800.015.1964282
GGTATCA29250.015.1794871
TATCGCC1954.1836756E-1115.17948733
TCTATCG1954.1836756E-1115.17948731
ATTAGAG3550.015.1126773
TTAGAGT1001.093432E-414.84
TAGCACC1001.093432E-414.84
TCTAGCG750.00410455814.79999928
CGCTAGA750.00410455814.79999937
GTACTAT2400.014.6458341
Produced by FastQC (version 0.11.5)