FastQCFastQC Report
Thu 28 Apr 2016
ERR1041894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1902311
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT65240.3429512839908932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54170.2847589064038425No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36870.19381688903654554No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA29810.15670413512827291No Hit
GAGTACGGGGGAGCCATTGTGGCTCCGGCCGGTTGCGCGGGCC19080.10029905730451014No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26100.032.4636041
GTATCAA38650.021.826652
CTAACGC656.90517E-519.9230773
GTACTAG656.90517E-519.9230771
TAGACTG902.1537253E-618.55
TGTACTA902.1537253E-618.55
TAATCCG609.239845E-418.55
TTAACGG752.0681637E-417.26666635
GTGTTAG4500.016.8555561
TCTATAC1151.2431647E-616.0869563
GTTAGCC4850.015.6391753
CTACCCT5600.015.1964294
CTATACT2450.015.102044
CATAGGG2450.015.102042
AGTGCGC1601.0975782E-815.03125110
ACGCCCT7700.014.8961044
TATCAAC57350.014.8064523
ACCTATA1001.09396184E-414.82
ACCGTGT2251.8189894E-1214.88
TATACAG1406.0003003E-714.5357155
Produced by FastQC (version 0.11.5)