FastQCFastQC Report
Thu 28 Apr 2016
ERR1041893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2434081
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT64840.26638390423326097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60630.24908784876099027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT40460.16622289890928035No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA27820.11429364922531336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26450.031.7542531
GTATCAA40800.020.5404422
CGTACGA500.007037086418.517
TAACGGC1502.5102054E-1017.26666636
GTATTAA1655.4569682E-1116.818181
TTAACGG1554.0199666E-1016.70967735
GTATTAG3450.016.6231881
GCGTAGA1751.3278623E-1015.8571431
TTTAGCG1059.351605E-615.85714226
CCCACGC10250.015.5219511
GTGTTAG4950.015.3232331
TTTAACG1701.4879333E-915.23529334
CGCTATA2800.015.1964282
GCTATAC1601.0981239E-815.031253
ATAGAAC1853.0559022E-1015.03
ACGCCCT10650.014.9389674
TACACAG4900.014.724495
CTAGACA2550.014.5098044
GTATAGA3100.014.322581
GGACCGT1956.730261E-1014.230776
Produced by FastQC (version 0.11.5)