FastQCFastQC Report
Thu 28 Apr 2016
ERR1041891.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041891.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2215586
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33620.15174315057054882No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT30600.1381124451950861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21350.031.8009361
TAAGTCG451.3237534E-424.6666685
GTATCAA29100.023.2044662
CTAGTAC1550.022.6774183
TAACGGC2000.022.236
TTAACGG1950.021.8205135
TTTAACG2150.019.79069734
TCTATAC1457.2759576E-1219.137933
ATTAGTA1156.412847E-817.6956522
TATCAAC41600.016.4098553
ATCAACG41750.016.3508994
TCAACGC41850.016.2676225
CAACGCA42150.016.1079486
GTGTAAG3150.015.8571431
CGCTATA2250.015.6222222
AACGCAG43900.015.5079727
GTTTAAC2750.015.47272833
TACGGGT3000.015.4166674
TATTAGA3850.015.3766232
ATTAGAG3500.015.3285713
Produced by FastQC (version 0.11.5)