FastQCFastQC Report
Thu 28 Apr 2016
ERR1041890.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041890.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4601588
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT69560.1511652064461225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT65660.142689871409609No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA49810.1082452405560863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT46310.1006391706515229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40000.031.218751
GTATCAA59750.020.713812
GTACCGT752.0689673E-417.2666666
CGCTATA5850.017.0769232
CTAGTAC1452.9867806E-916.5862063
TTTAGCG3550.016.1549326
TCGCTAT7100.015.6338031
TCTATAC2500.015.543
TAGACTA1601.0990334E-815.031255
GTATAAG4350.014.8850581
TTAGACA2500.014.7999994
TCTGTCG8200.014.6646348
TCAACGC84850.014.6517385
TATCAAC85600.014.6314253
ATCAACG84850.014.6299354
GTACGTA1904.5656634E-1014.60526413
TTAGACT2700.014.3888894
CAACGCA86000.014.3697676
GCTATAT7400.014.253
TATATTG8150.014.0736195
Produced by FastQC (version 0.11.5)