FastQCFastQC Report
Thu 28 Apr 2016
ERR1041889.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041889.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2339342
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49370.21104225034219026No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44740.1912503601440063No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31260.13362731913503884No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA28640.12242758861252438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23650.029.4904861
GTATCAA33950.020.4344622
CGATCGT555.1452813E-420.18181826
TATACAC2150.018.9302333
GTATTAG2550.018.8627451
CGTTAAC609.2407153E-418.51
CTAGTAC1258.591087E-917.763
TATACTG2100.017.6190475
TCTATAC1603.45608E-1117.343753
TAGACAG2700.016.4444455
GTGTTAG4300.015.9186041
TAAACGT700.002593568815.8571434
TTAACGG1302.5921145E-715.65384635
GTATATA1302.5921145E-715.6538461
CTACACT4050.015.5308644
CTATACT2300.015.2826094
TGTACCG1101.4530671E-515.1363645
TACGCTG1601.0981239E-815.031255
CTAGACA2350.014.9574474
TATAGAC750.00410609514.83
Produced by FastQC (version 0.11.5)