FastQCFastQC Report
Thu 28 Apr 2016
ERR1041888.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041888.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences975377
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT21880.22432351798330288No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20100.20607416414371058No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA16870.17295876363703472No Hit
CCCACGCCCTCTGCATCTTTTTTCTTTTCCCCCACGGATCTTT15230.1561447522342643No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT13900.14250899908445658No Hit
ATGTGGACCAGGTTGGCCTCGAACTCGTGCCCTCGAACCCTCG11250.11534001724461412No Hit
CATGTGGACCAGGTTGGCCTCGAACTCGTGCCCTCGAACCCTC11100.11380215034801927No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11350.032.5991171
GCGAATA250.00549594129.629
GTATCAA17250.021.6637672
TATACCG806.957889E-720.81255
TATACTG555.142327E-420.1818185
CTTGGAC2200.019.3409083
TCTCGTA1254.129106E-1019.2428
TCGTATG1254.129106E-1019.2430
TATACAC1254.129106E-1019.243
CTCGTAT1306.9667294E-1018.529
GTATTAT701.21895755E-418.51
GTAATAC609.2354504E-418.53
TTATACT500.007034137418.54
TACATAC1002.8738214E-718.53
GTATGTA801.616261E-518.51
ATATACC701.21895755E-418.53
TAGGACG500.007034137418.54
TTACACT701.21895755E-418.54
ATTCTAG500.007034137418.51
GTGTTAG2650.018.1509441
Produced by FastQC (version 0.11.5)