FastQCFastQC Report
Thu 28 Apr 2016
ERR1041887.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041887.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4407169
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA80670.18304267433356877No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60260.13673176590232866No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57190.12976584288008922No Hit
CATGTGGACCAGGTTGGCCTCGAACTCGTGCCCTCGAACCCTC50780.11522135865450134No Hit
ATGTGGACCAGGTTGGCCTCGAACTCGTGCCCTCGAACCCTCG50680.11499445562446096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.032.057431
GTATCAA60650.021.0164892
GTATTAG7550.017.6423841
GTATATA1603.45608E-1117.343751
TTAGACT1502.5283953E-1017.2666664
TACATAC3900.017.0769233
TGTACTA1757.2759576E-1216.9142865
GTATTAA1251.6607373E-716.2799991
TATTAGA6500.016.2230762
TAATACG2200.015.9772734
GTATAGA3950.015.924051
CCTCTAG6550.015.8167931
TTAACGG5500.015.80909135
CCTAGTA4450.015.7977522
CGCTATA5400.015.7592582
TATACTG3100.015.5161295
ATTAGAG6200.015.2177413
GTACTAA1001.09448134E-414.7999991
TATCGTT1001.09448134E-414.79999918
AGTACTG7500.014.7999995
Produced by FastQC (version 0.11.5)