FastQCFastQC Report
Thu 28 Apr 2016
ERR1041886.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041886.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15196174
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT243440.16019821831468894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT219900.1447074770267832No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT159060.10467108365566227No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA104200.029.0993271
GTATCAA157350.019.1525272
TTAACGG11650.017.46781235
GTATTAG28750.016.1513041
TTTAACG13400.015.60074634
CTAGTAC8600.015.0581393
TATACTG18300.014.8606555
TAACGGC14050.014.48398536
TTATACG10550.014.02843635
TCGCTAA7400.014.014
TATACGA10400.013.87536
TCAACGC222000.013.5583335
ATCAACG221350.013.5563594
GTATACG4400.013.4545451
CAACGCA225450.013.3262376
CTACACT23800.013.2920164
TATCAAC227650.013.2787173
TATTAGA22600.013.1792042
TTAGACT12050.013.0497924
CTAATAC29000.013.0137933
Produced by FastQC (version 0.11.5)