FastQCFastQC Report
Thu 28 Apr 2016
ERR1041885.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041885.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15670030
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT302890.1932925463448379No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT289280.1846071768847922No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT203070.12959132815955043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA111050.029.5866721
GTATCAA174950.018.653332
GTATTAG28900.015.5553641
CGCTATA8100.015.5308642
TCTTATA51950.014.28007737
TTAACGG11300.014.24336335
TCGCTAA7700.013.69480514
TAGACTG19250.013.4545465
GTATACG5150.013.2912631
TCAACGC247500.013.2303035
TTATACG10350.013.22705435
ATCAACG247100.013.2142864
CCTATAC22200.013.03
CAACGCA252850.012.9357336
TATCAAC255800.012.9167333
CTTATAC33850.012.8980837
CTAATAC32450.012.7704163
TATTAGA25850.012.7388782
TAACGGC12700.012.67322836
GTATAGA24250.012.6639181
Produced by FastQC (version 0.11.5)