FastQCFastQC Report
Thu 28 Apr 2016
ERR1041884.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041884.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17205368
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT252690.14686695454581383No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235740.13701537799133387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105100.029.0789721
GTATCAA160800.018.9947152
TTAACGG12900.016.06201635
GTATTAG36300.015.5440771
TTTAACG15800.014.40189834
TATACTG22350.014.2371375
TCTATAC16050.014.177573
TCGCTAA9650.013.611414
TAACGGC15400.013.45454536
TATACGA12050.013.35684836
TTAGACT13900.013.3093534
TTAGCGA10300.013.29126327
ATCAACG228000.013.290794
TCAACGC227950.013.2612415
TTATACG11950.013.15899735
CAACGCA229700.013.1279936
TATCAAC235600.013.1054743
TTTAGCG13150.013.08365126
CTACACT28150.012.8809954
TACACTG35500.012.8718325
Produced by FastQC (version 0.11.5)