FastQCFastQC Report
Thu 28 Apr 2016
ERR1041883.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041883.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10635934
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185500.17440875432284556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT160640.1510351606168297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT114060.1072402292078909No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA71600.029.8687131
GTATCAA107700.019.7024142
TCGCTAA9400.018.30319214
TTTAGCG10000.017.7626
TTAACGG10500.016.56190535
CTCGCTA10600.015.88207513
GTATTAG30350.015.5436571
TTAGACT9050.015.3314914
TAACGGC11550.015.21645136
TATACGA9850.015.21319936
TTTAACG12850.014.97276334
ATTAGAC7050.014.9574473
TTATACG10050.014.72636835
GTACAAG24850.014.5915491
GTATAGG16450.014.5075981
TATACTG11500.014.4782615
TATTAGA20950.014.2171852
TTAGCGA11150.013.9372227
TCTATAC10800.013.7037033
CGCCTTA12350.013.63157924
Produced by FastQC (version 0.11.5)