FastQCFastQC Report
Thu 28 Apr 2016
ERR1041882.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041882.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17424228
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT315100.18084014970419351No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT305050.17507231884247612No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT234270.1344507199974656No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA106900.028.5720311
GTATCAA170350.017.810392
TATACTG30900.016.1051795
GTATAGG26550.014.6327681
TCTATAC21350.014.5573773
GTATTAG40350.014.3965291
CGCTATA6850.014.31386852
TTAACGG11700.013.91453135
TACACTG44050.013.6492615
TCTTATA72850.013.35758437
CTTATAC46950.013.04259937
CTACACT31850.012.9529054
ATCAACG237000.012.778274
TAACGGC12600.012.77380936
TCAACGC237050.012.7677715
GAGTACG236000.012.5737291
TATCAAC245150.012.4817473
CAACGCA242650.012.4578626
TATACCG12350.012.4331985
AGTACGG236950.012.328132
Produced by FastQC (version 0.11.5)