FastQCFastQC Report
Thu 28 Apr 2016
ERR1041881.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041881.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15501170
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238150.15363356443416853No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT225920.14574383740066074No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT158700.10237936878313056No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96650.029.707191
GTATCAA151050.018.824562
GTATTAG35300.016.1416441
TTAACGG13500.015.62222235
TATACTG20500.015.4317065
TCTATAC16750.015.1313433
TTATACG12400.015.06854935
CTAGTAC8450.014.668643
TATACGA13050.014.45976936
TAACGGC15050.014.38206136
GTACTAG7850.013.9044591
GTATAGA23250.013.8451621
TTAGCGA10700.013.83177627
TTTAACG16050.013.83177534
GTATACG4950.013.8282831
TATATCG7350.013.5918365
ATCAACG213350.013.3102884
TCAACGC213950.013.2643155
CTAATAC32050.013.16068653
CAACGCA216600.013.1105736
Produced by FastQC (version 0.11.5)