FastQCFastQC Report
Thu 28 Apr 2016
ERR1041880.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041880.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18218620
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT434820.23866791227875658No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT405060.2223329758236354No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT359530.19734205993648257No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145650.028.6295911
CTTATAC89950.020.66981737
GTATCAA236600.017.5929852
TCTTATA170150.017.5377637
TCGCTAA9750.016.3179514
GTATTAG28950.015.0811741
TATACCG11100.014.5000015
TAATACT32300.013.5170284
CCTATAC19850.013.2342573
CGCTATA7600.013.1447372
GTATAGA23100.013.1341991
TATACTG22600.012.9336285
CTAATAC31650.012.8594013
TAGACTG20850.012.688255
CTCTTAT262800.012.66419437
GTGTAAG28950.012.3972361
ATCAACG340150.012.2209324
TATCAAC344000.012.1863373
TCAACGC343400.012.1214325
CAACGCA346100.012.0001446
Produced by FastQC (version 0.11.5)