FastQCFastQC Report
Thu 28 Apr 2016
ERR1041879.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041879.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences393307
Sequences flagged as poor quality0
Sequence length43
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7270.18484288354898337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7270.18484288354898337No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT5110.12992395253580538No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA4600.11695698271324945No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4350.032.321841
CTATACT303.5952125E-430.8333324
TATACTG303.5952125E-430.8333325
AGCTTAA250.005492002729.610
GCTATAC250.005492002729.63
CGGATAA453.9994484E-628.77777725
TAGGACA358.858981E-426.4285724
TTATACA901.4188117E-1024.6666662
TATACAC901.4188117E-1024.6666663
CTATTCT400.001928941323.1254
CACCGTT400.001928941323.1257
TTCACGC903.808964E-922.6111122
GTATCAA6300.022.023812
CTAACAC705.0878534E-621.1428593
CTTATAC1059.767973E-1021.1428571
TGTATAA450.00382140220.55555514
TACGGGT450.00382140220.5555554
GTGTATA450.00382140220.55555513
GTATAAG450.00382140220.55555515
ATACACA1101.7444108E-920.1818184
Produced by FastQC (version 0.11.5)