FastQCFastQC Report
Thu 28 Apr 2016
ERR1041877.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041877.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15072071
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT212190.14078357247653625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT203620.13509755892206185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA103650.030.217561
GTATCAA156650.019.8640292
GTATTAG14450.015.1072661
TATACTG15350.014.9446255
GTGTTAG29250.014.1675221
TAGACAG20150.013.7717125
GTATTAA11300.013.5884951
GTATAGA13350.013.4419481
TATCAAC233600.013.3839893
ATCAACG232550.013.3728234
TCAACGC233150.013.3542785
TCTAGAC12450.013.0763053
CAACGCA237600.013.0730226
CTAGACA15200.013.0230264
TTAGACA11200.012.8839294
TCGCTAA4350.012.7586214
AACGCAG245950.012.6968917
CCTATAC11600.012.5991383
GTCTTAC12050.012.5892111
CTACACT21300.012.4201894
Produced by FastQC (version 0.11.5)