FastQCFastQC Report
Thu 28 Apr 2016
ERR1041876.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041876.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7710101
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158280.20528913953267278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT148760.19294170076371242No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT102050.132358836803824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA69550.029.099931
GTATCAA101650.019.8376772
TATACTG8850.017.97745
CGCTATA8550.017.0935672
CTATACT6600.015.696974
TCGCTAT10550.014.55450251
GTATAGA6400.014.1640631
GTATACT4050.014.1604944
TAGACAG10200.014.14705855
TCTTATA22800.013.95614137
GTCCTAA6650.013.6315791
TATCAAC150600.013.5494683
ATCAACG149600.013.5163774
TCAACGC151100.013.3454675
CAACGCA151700.013.3170736
GTATTAG6000.013.2583331
CTACACT9100.013.2142854
GTCTTAG6200.013.1290321
GTCTATA5100.013.0588241
TAATACT7250.013.0137934
Produced by FastQC (version 0.11.5)