FastQCFastQC Report
Thu 28 Apr 2016
ERR1041875.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041875.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7271913
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT124760.17156420875772302No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT113070.15548865889897198No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT80270.11038360882480304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.029.7283231
GTATCAA78300.019.6104722
CGCTATA10900.015.4449542
TTATACG4400.015.13636435
GTGTTAG19500.014.81
TATACGA4450.014.55056336
GTATACG2301.8189894E-1214.4782611
TATCAAC112050.013.7697463
GTATTAG8200.013.7621951
GTCGCTA5850.013.5982937
ATCAACG112150.013.5430224
TAGATAG3850.013.4545455
TCGCTAT12800.013.4414061
TATATCG1805.1715688E-813.3611115
TCAACGC114000.013.2907895
TATACTG9100.013.2142855
GTCTATA5650.013.0973451
CAACGCA117050.012.9602736
GTATAAG7150.012.9370631
TAGACTC5900.012.8559325
Produced by FastQC (version 0.11.5)