FastQCFastQC Report
Thu 28 Apr 2016
ERR1041874.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041874.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2337170
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT34280.14667311320956541No Hit
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA33970.14534672274588498No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29300.1253652922123765No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17150.030.9591831
TATCGCG502.7032604E-422.27
GTATACG450.003826973320.5555571
GTATCAA25900.020.2857152
TCGCTAA801.6178352E-518.514
GTCTTAC1409.458745E-1118.51
TATACGG609.240711E-418.52
CGCTATA2450.017.3673462
TGTATAC1452.9849616E-916.5862063
GTCGCTA1251.6597551E-716.27999937
GTATATA1251.6597551E-716.2799991
GTGTTAG4550.016.2637351
CGCTAAG700.002593569515.8571421
GTGTAAG2600.015.6538461
TTAGACT957.064667E-515.5789484
TAGACTG1802.0190782E-1015.4166675
TCTGTCG6700.015.1865668
TAGACAC1954.1836756E-1115.1794865
TCGCTAT3050.015.1639351
TACACAG4350.014.8850585
Produced by FastQC (version 0.11.5)