FastQCFastQC Report
Thu 28 Apr 2016
ERR1041873.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041873.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16464251
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT204560.12424494743186314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT201370.12230741623168889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.027.0446621
GTATCAA139450.017.7239152
TTAACGG12850.016.12451435
CGCTATA9200.015.2826092
TTTAACG14200.015.24295734
GTATTAG35750.014.6965041
TATACTG22900.014.4606995
TCTATAC18450.014.0379413
TTTAGCG10550.014.02843726
GTACTAG9650.013.9948181
TAACGGC15300.013.78431336
TTATACG11950.013.77824335
TATACGA12050.013.663936
GTATAGG23500.013.4617021
CTACACT26650.012.9812384
TATATCG7050.012.8581565
GAGTACG250450.012.8159321
CTAGTAC9400.012.7925523
CTAATAC32850.012.7275493
AGTACGG251050.012.7263482
Produced by FastQC (version 0.11.5)