FastQCFastQC Report
Thu 28 Apr 2016
ERR1041871.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041871.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17825941
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT227410.12757250795343708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT223430.12533980674568598No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105250.029.3363421
GTATCAA159900.019.1131952
CGCTATA11200.014.5357152
TATACCG8200.014.2134155
TTAACGG10100.013.92079335
GTATTAG27150.013.7642731
GTATAGG19150.013.7180151
TCTAGAC18300.013.4453543
ATCAACG227000.013.3982374
TATCAAC229850.013.3930823
TCAACGC227100.013.3434625
CTAGTAC9500.013.24210553
CAACGCA232100.013.0799226
CCTATAC19450.012.8406173
TAATACT30250.012.7818184
CTAATAC28050.012.7290543
GTACTAG9450.012.7248681
TAGACAG31600.012.7041145
AACGCAG242300.012.6132917
CTACACT29250.012.4598294
Produced by FastQC (version 0.11.5)