FastQCFastQC Report
Thu 28 Apr 2016
ERR1041869.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041869.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8699362
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108560.12479076051784027No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT104930.12061804072528537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54950.029.424931
GTATCAA82500.019.5315132
CGCTATA6700.016.0149252
GTATTAG11700.015.4957271
TATACCG3550.015.1126765
CTAGTAC4450.014.9662933
GTGTTAG17550.014.3361831
TATACTG9500.014.215795
TATACGA3800.014.11842236
GTCTTAG10350.013.9420291
TTAGACA7350.013.8435374
TATCAAC116850.013.8373993
ATCAACG117250.013.7270794
GTATAGG7350.013.5918371
TCAACGC118850.013.5267135
CAACGCA119750.013.4096036
GTATACT7500.013.0733344
GTCTAAG5100.013.0588251
CTAACGC2551.6370905E-1113.0588253
TAGACAG13150.012.94296555
Produced by FastQC (version 0.11.5)