FastQCFastQC Report
Thu 28 Apr 2016
ERR1041868.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041868.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3756569
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT55990.14904557855851974No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53100.1413523883096517No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30600.029.7450981
GTATCAA44050.020.494892
GTATTAA2000.019.4250011
TAAACGG500.00703778118.55
GTACTAG1601.8189894E-1218.51
TATTCCG852.7257802E-517.4117645
CGCTATA2750.016.8181822
TATACAC3850.016.3376623
TTATACG1504.6802597E-916.03333335
GTATATA1851.8189894E-1116.01
TTAGCGA2201.8189894E-1215.13636427
TTATGAC2500.014.7999993
CTAATAC4650.014.322583
ACGCCCT16250.014.2307694
TTAGACT1304.4501376E-614.2307694
TAGACTG2750.014.1272725
GTATTAG5250.014.0952381
TTATACA4200.014.0952382
TTAATAC1458.9246896E-714.0344833
GTTAATA2900.014.0344832
Produced by FastQC (version 0.11.5)