FastQCFastQC Report
Thu 28 Apr 2016
ERR1041866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2597138
Sequences flagged as poor quality0
Sequence length43
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTCAGAGAGGCGAGGGTTCGAGGGCACGAGTTCGAGGCCAA40520.1560178935428152No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18200.029.1730791
TTAGCGT751.3755198E-824.6666664
TTAGTAC400.00193183923.1253
CTAGTAC806.967075E-720.81253
GTATCAA26050.020.026872
GTCCTAA1355.638867E-1119.1851861
TAGTACT1551.8189894E-1219.0967734
GTTAACC500.0070372118.53
TATAGAC1301.3953468E-817.0769233
GTTCTAA2200.016.8181821
TATACTG1352.222805E-816.4444455
CTGTATA700.002593672615.8571434
TCTTATA3850.015.85714237
GTCTTAG2003.6379788E-1215.7251
TAATACA1302.592442E-715.6538464
TTCTAGA2250.015.6222222
TAACACT1902.7284841E-1115.5789484
TATACAC2055.456968E-1215.3414643
CTAGACG855.3673843E-415.2352934
GTATTAG1701.4879333E-915.2352931
Produced by FastQC (version 0.11.5)