FastQCFastQC Report
Thu 28 Apr 2016
ERR1041865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1041865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6340970
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT196870.31047300334176003No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT177120.2793263491232414No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT126640.19971707798649105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA77600.030.7538661
GTATCAA118250.020.181822
CTAGTAC4200.018.53
CCTAGTA6050.016.2066122
GTCTAAG4750.015.9684211
TACACTG11950.015.6359835
TATACTG7750.015.277425
TAATACT9150.015.1639354
GTACTAG3450.015.0144921
CTAATAC8450.014.4497033
GTATTAG7450.014.1543621
CTACACT8950.014.0558654
TAGACAG8650.013.9017355
TATCAAC174100.013.8032743
ATCAACG174750.013.7307594
TTATACG1501.3080498E-613.56666735
TAGTACT7800.013.5192314
GTATACG3150.013.5079371
TCAACGC177000.013.4935035
GTACTGT11250.013.4844456
Produced by FastQC (version 0.11.5)